The detection of feline herpesvirus-1 (FHV-1) using polymerase chain reaction (PCR).

Detection of Babesia ovis Using Polymerase Chain Reaction.

Complete genomic sequence and an infectious BAC clone of feline herpesvirus-1 (FHV-1).

Infection with feline herpesvirus-1 (FHV-1) is a major cause of upper respiratory and ocular diseases in Felidae. We report the first complete genomic sequence of FHV-1, as well as the construction and characterization of a bacterial artificial chromosome (BAC) clone of FHV-1, which contains the entire FHV-1 genome and has the BAC vector inserted at the left end of U(L). Complete genomic sequen...

Detection of Chlamydia trachomatis Using polymerase chain reaction (PCR)

Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...

abrin gene detection, using polymerase chain reaction (pcr)

abrin known as ribosome inactivating protein’s (rip) it is a high cytotoxic plant protein. high lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. as a result, remaini...

Polymerase chain reaction (PCR) for the detection of herpesvirus in tortoises.

A polymerase chain reaction (PCR)-based method using a herpesvirus consensus primer was assessed for the identification of herpesviral infections in tortoises. A single band of about 230 bp was detected in PCR products from two out of twenty swabs taken from the oral cavity, three out of three paraffin-embedded tissue sections from the liver (two cases) and oral mucosa (one case), and one out o...